This technical exchange workshop focuses on the use of environmental DNA (eDNA) for the surveying and monitoring of macrofaunal communities. While the focus currently lies on aquatic ecosystems there are emerging approaches for the use of barcoding from soil, wood and other sources. In particular, the workshop seeks to provide an opportunity for practitioners to come together to discuss new developments or challenges with the “nuts and bolts” of eDNA applications and to learn from each other. The workshop will have a highly applied flavor discussing ongoing DNA monitoring projects. We will highlight the workflow of a typical eDNA project and also discuss how citizen science approaches can be combined for large scale genetic monitoring and assessing biodiversity.